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KMID : 0355620080340010049
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Journal of Korean Association of Oral and Maxillofacial Surgeons
2008 Volume.34 No. 1 p.49 ~ p.58
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The BMPs expression and histomorphometric study of ?-TCP / rhBMP-2 Grafting on the rabbit cranial bone defects
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Lim Byung-Sup
Jeon Jae-Yun
Park Chang-Joo
Im Jae-Jung
Hwang Kyung-Gyun
Shim Kwang-Sub
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Abstract
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Objective: The Purpose of the study was to investigate the bone morphogenic protein expression of rhBMP-2(recombinant human bone morphogenic protein-2) as singnaling molecule and ?-TCP(Tricalcium phosphate) as a bone substitute and carrier medium of rhBMP-2.
Materials and Methods: 16 rabbits divided into 2 group of each 8 rabbit. Two standardized bone defect, round bilateral defect was made in the cranium of the 8 rabbit of first group, and was grafted with 150~500?m diameter ?-TCP 0.25g in one side, which was soaked with rhBMP-2, and autogenous bone was grafted on another side as a positive control. Second group of 8 rabbit, only ?-TCP was grafted with same size and same manner. After 2, 4, 8, and 12 weeks, specimen was taken for microscopic immunohiostochemical and histomorphometric analysis.
Result: Grafting ?-TCP with rhBMP show the early formation of the bone regenerative factor (BMP-4) and more quantity of new bone formation than only use of ?-TCP (8,12 week), even show less new bone formation than autogenous bone.
Conclusion : The experimental study result that ?-TCP graft combination with rhBMP-2 as a delivery system is an effective with osteoinductive capacity and biodegradable properties, so that provide clinical availibility of composite use in reconstruction of bony defect.
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KEYWORD
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Beta-Tricalcium phosphate, bone graft, Bone Morphogenic Protein, bone substitute, histomorphometry, Immunohistochemistry
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